This course will cover the following topics:

SARS-CoV-2 GENE EXPRESSION

1. Introduction

2. RNA Dependent RNA Polymerase Function

3. RNA Dependent RNA Polymerase Structure

4. Transcription Regulatory Sequence

5. Discontinuous Transcription – 1

6. Discontinuous Transcription – 2

7. Translation of RNA

8. Translation of Subgenomic RNA

REPLICATION AND TRANSCRIPTION COMPLEXES

1. What are RTCs

2. Formation of RTCs

3. Pattern Recognition Receptors

4. Viral Cytoplasmic Sensors, and

5. Benefits of RTCs

RTPCR – RNA EXTRACTION

1. Introduction

2. Components

3. Lysis Buffer

4. Silica Membrane Based RNA Extraction Part 1

5. Silica Membrane Based RNA Extraction Part 2

6. Magnetic Bead Based RNA Extraction Part 1

7. Magnetic Bead Based RNA Extraction Part 2

CHEST X RAY IN COVID 19

1. Resources

2. Imaging recommendations

3. Interpretation of CXR report

4. Probable COVID 19 CXR

5. Non-COVID 19 CXR

6. Baseline CXR in COVID 19

D-DIMER

1. Introduction

2. D-dimer levels

3. Etiology of pulmonary embolism

4. Risk Factors for Pulmonary Embolism

5. Approach to Pulmonary Embolism

6. D-dimer in COVID-19

ROLE OF VACCINES IN COVID 19

1. Introduction

2. What is vaccine Efficacy?

3. Calculation of Efficacy

4. What is Herd Immunity?

5. Vaccine & Herd Immunity

6. What are Variants?

7. Vaccines & Variants

8. Vaccine Protection

9. Vaccine & GAVI

PFIZER BIONTECH VACCINE

1. Introduction

2. About Pfizer

3. About BioNTech

4. Vaccine Efficacy

5. Vaccine Administration

6. Vaccine Side-effects

7. Vaccine related Precautions

PRINCIPLES OF VIRAL VECTOR VACCINES

1. Introduction to adenoviruses

2. Lifecycle of adenoviruses

3. Modification of adenoviruses

4. How do they function?

5. Viral vector vaccines

6. Advantages of adenoviruses